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Cat No.
DIA-199
Description
In enzymology, a glycerol-3-phosphate oxidase (EC 1.1.3.21) is an enzyme that catalyzes the chemical reaction: sn-glycerol 3-phosphate + O2 ? glycerone phosphate + H2O2. Thus, the two substrates of this enzyme are sn-glycerol 3-phosphate and O2, whereas its two products are glycerone phosphate and H2O2. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. This enzyme participates in glycerophospholipid metabolism. It employs one cofactor, FAD.
Abbr
L-α-glycerophosphate oxidase (Pediococcus sp.)
Species
Pediococcus sp.
Enzyme Commission Number
EC 1.1.3.21
Bio-activity
GradeⅢ 40 U/mg-solid or more (containing approx. 40% of stabilizers)
Appearance
Yellowish amorphous powder, lyophilized
Molecular Mass
approx. 76 kDa (by gel filtration)
pH Stability
pH 6.5-8.5 (25°C, 20hr)
Michaelis Constant
3.2×10?3M (L-α-Glycerophosphate), 6.8×10?3M (D, L-form)
Isoelectric point
4.1±0.1
Specificity
The enzyme has the highest specificity for L-form of α-glycerophosphate.
Optimum pH
8.0-8.5
Optimum temperature
35-40°C
Stabilizers
Sucrose, FAD
Thermal stability
below 40°C (pH 7.0, 15min)
Stability
Stable at-20°C for at least one year (A decrease in activity of ca. 10% may occur at 5°C within 6 months.)
Inhibitors
Ionic detergents (SDS, LBS, etc.), Hg??, Ag?
Applications
This enzyme is useful for enzymatic determination of triglyceride when coupled with lipoprotein lipase and glycerokinase in clinical analysis.
Synonyms
L-α-glycerophosphate oxidase; sn-glycerol-3-phosphate: oxygen 2-oxidoreductase; glycerol phosphate oxidase; glycerol-1-phosphate oxidase; glycerol phosphate oxidase; L-alpha-glycerophosphate oxidase; alpha-glycerophosphate oxidase; L-alpha-glycerol-3-phosphate oxidase; EC 1.1.3.21
