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Cat No.
DIA-210
Description
Lipoprotein lipase (LPL) (EC 3.1.1.34) is a member of the lipase gene family, which includes pancreatic lipase, hepatic lipase, and endothelial lipase. It is a water soluble enzyme that hydrolyzes triglycerides in lipoproteins, such as those found in chylomicrons and very low-density lipoproteins (VLDL), into two free fatty acids and one monoacylglycerol molecule. It is also involved in promoting the cellular uptake of chylomicron remnants, cholesterol-rich lipoproteins, and free fatty acids. LPL requires ApoC-II as a cofactor.
Abbr
LPL (Pseudomonas sp.)
Alias
LPL
Species
Pseudomonas sp.
Enzyme Commission Number
EC 3.1.1.34
Bio-activity
GradeⅢ 20U/mg-solid or more (containing approx. 80% of stabilizers)
Appearance
Light brown amorphous powder, lyophilized
Molecular Mass
approx. 134 kDa
pH Stability
pH 7.0-9.0 (25°C, 20hr)
Isoelectric point
5.95±0.05
Optimum pH
7.0-9.0
Optimum temperature
45-50°C
Stabilizers
Mg??, Na-cholate, bovine serum albumin
Thermal stability
below 55°C (pH 7.0, 10min)
Stability
Stable at-20°C for at least one year
Inhibitors
Hg??, Ag?, ionic detergents
Applications
This enzyme is useful for enzymatic determination of triglyceride in serum when coupled with L-α-glycerophosphate oxidase and glycerol kinase. Usually, the reaction can be completed in 5 minutes at 37°C by using 2.5~3.0 units of the enzyme per test (3.0ml) at pH around 7.0.
Synonyms
Lipoprotein lipase; LPL; EC 3.1.1.34; Clearing factor lipase; Diacylglycerol lipase; Diglyceride lipase
