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Cat No.
DIA-273
Abbr
Neutral proteinase (Bacillus sp.)
Species
Bacillus sp.
Enzyme Commission Number
EC 3.4.24.4
Bio-activity
1000U/mg-solid or more
Appearance
White amorphous powder or granule
Molecular Mass
approx. 25,000 (SDS-PAGE)
pH Stability
pH 6.0-9.0 (40°C,30min)
Unit Definition
One unit causes an increase in the optical density at 275nm corresponding to one microgram of tyrosine per minute under the conditions described below.
Optimum pH
pH 7.0-8.0
Optimum temperature
80°C
Stabilizers
Ca??
Thermal stability
below 70°C (pH 7.5, 10min)
Stability
Stable at 5°C for at least one year
Inhibitors
EDTA
Applications
This enzyme is useful for synthesis of oligopeptides, for proteolysis of insoluble protein and for structural investigation of the protein.
Synonyms
Neutral proteinase; Cathepsin G; Microbial metalloproteinase; EC 3.4.24.4
