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Cat No.
DIA-163
Description
Phospholipase C is an enzyme with system name phosphatidylcholine cholinephosphohydrolase. This enzyme catalyses the following chemical reaction: a phosphatidylcholine + H2O? 1, 2-diacyl-sn-glycerol + phosphocholine. The bacterial enzyme is a zinc protein. It also acts on sphingomyelin and phosphatidylinositol.
Abbr
Phospholipase C (Bacillus cereus)
Species
Bacillus cereus
Form
Freeze dried powder
Enzyme Commission Number
EC 3.1.4.3
Bio-activity
> 30 U/mg
Reaction
a phosphatidylcholine + H2O ? 1,2-diacyl-sn-glycerol + phosphocholine
Appearance
White to brownish powder
pH Stability
6.5-9.0
Optimum pH
7.0-9.0
Thermal stability
Stable at 60°C and below (pH 7.5, 10 mins)
Applications
Useful for enzymatic determination of lecithin
Synonyms
Phospholipase C; EC 3.1.4.3; lipophosphodiesterase I; Clostridium welchii alpha-toxin; Clostridium oedematiens beta-and gamma-toxins; lipophosphodiesterase C; phosphatidase C; heat-labile hemolysin; alpha-toxin
