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Cat No.
DIA-415
Description
The enzyme urate oxidase (UO), or uricase or factor-independent urate hydroxylase, absent in humans, catalyzes the oxidation of uric acid to 5-hydroxyisourate: Uric acid + O2 + H2O → 5-hydroxyisourate + H2O2 → allantoin + CO2
Abbr
UO, Recombinant (E. coli)
Alias
UO
Source
E. coli
Species
E. coli
Enzyme Commission Number
EC 1.7.3.3
Bio-activity
> 4 U/mg lyophilizate
Appearance
Light brownish lyophilizate
Molecular Mass
ca. 90 kDa
pH Stability
7.0–11.0
Michaelis Constant
1.1 x 10^-5 M (uric acid)
Structure
2 subunits of 35 kDa (SDS-PAGE)
Unit Definition
One unit (U) is defined as the amount of enzyme which oxidizes 1 μmol of uric acid per min at 25°C and pH 8.5.
Optimum pH
8.5
Optimum temperature
45°C
Stabilizers
Citrate, sucrose
Thermal stability
below 55°C
Stability
Stability (liquid form) stable at 37°C for at least ten days
Stability (powder form) stable at 30°C at least three weeks
Inhibitors
Hg2+, Ag+
Synonyms
urate oxidase; uric acid oxidase; uricase; uricase; urate: oxygen oxidoreductase; EC 1.7.3.3; uricase II
