Cat No.
DIA-276
Description
The enzyme urate oxidase (UO), or uricase or factor-independent urate hydroxylase, absent in humans, catalyzes the oxidation of uric acid to 5-hydroxyisourate: Uric acid + O2 + H2O → 5-hydroxyisourate + H2O2 → allantoin + CO2
Abbr
Uricase (Bacillus sp.)
Alias
UO; uricase
Species
Bacillus sp.
Bio-activity
GradeⅡ 1.5U/mg-solid or more
Appearance
White amorphous powder, lyophilized
Molecular Mass
approx.150,000
pH Stability
pH 6.0-9.5 (25°C, 20hr)
Michaelis Constant
1.36×10??M (Uric acid)
Structure
4 subunits per molecule
Isoelectric point
4.7
Unit Definition
One unit causes the oxidation of one micromole of uric acid per minute under the conditions described below.
Optimum pH
8.5
Optimum temperature
45°C
Stabilizers
Borate, EDTA, nonionic detergents
Thermal stability
below 60°C (pH 8.0, 10min)
Stability
Stable at -20°C for at least 6 months
Inhibitors
Ag?,Hg??
Applications
This enzyme is useful for enzymatic determination of uric acid in clinical analysis.
Synonyms
urate oxidase; uric acid oxidase; uricase; uricase; urate: oxygen oxidoreductase; EC 1.7.3.3; uricase II