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Cat No.
DIA-174
Description
The enzyme urate oxidase (UO), or uricase or factor-independent urate hydroxylase, absent in humans, catalyzes the oxidation of uric acid to 5-hydroxyisourate: Uric acid + O2 + H2O → 5-hydroxyisourate + H2O2 → allantoin + CO2
Abbr
UO (Jack bean)
Alias
UO; uricase
Species
Jack bean
Form
Freeze dried powder
Enzyme Commission Number
EC 1.7.3.3
Bio-activity
GradeⅡ(-201) 100U/mg-solid or more
CAS No.
9002-1 2-4
Appearance
White amorphous powder, lyophilized
Molecular Mass
approx. 480 kDa
pH Stability
pH 5.5-8.5 (30°C, 17hr)
Michaelis Constant
1.05×10?2M (Urea)
Structure
8 active sites with SH-groups per mole of the enzyme
Isoelectric point
5.0-5.1
Optimum pH
6
Optimum temperature
60°C
Stabilizers
EDTA, glutathione, succinate, BSA
Thermal stability
below 50°C (pH 8.0, 60min)
Stability
Store at-20°C (A decrease in activity of ca.15% may occur within 6 months)
Inhibitors
Heavy metal ions (Ag?,Hg??,etc.)
Applications
This enzyme is useful for enzymatic determination of urea in clinical analysis.
Synonyms
urate oxidase; uric acid oxidase; uricase; uricase; urate: oxygen oxidoreductase; EC 1.7.3.3; uricase II
