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Cat No.
NATE-0005
Description
In mammalian systems, β-hydroxybutyrate dehydrogenase is localized on the inner mitochondrial membrane and requires phosphatidyl choline for activity. In contrast, the enzyme from Pseudomonas is a soluble cytosolic enzyme that does not require a phospholipid allosteric activator. The enzyme is required for the utilization of ketone bodies as a source of metabolic energy. It catalyzes the oxidation of 3-hydroxybutyrate to acetoacetate, the first step in the conversion of ketone bodies to citric acid, which is then further metabolized via the tricarboxylic acid cycle (Krebs cycle).
Abbr
3-HBDH, Native (Alcaligenes faecalis)
Alias
3-HBDH
Species
Alcaligenes faecalis
Form
Freeze dried powder
Enzyme Commission Number
EC 1.1.1.30
Bio-activity
> 1,500 U/mg
Appearance
White powder
Molecular Mass
60±5 kDa (TSK G?3000SW); 30±5 kDa (SDS?PAGE)
pH Stability
5.5-11.0 (37°C, 60 mins)
Michaelis Constant
D?3?Hydroxybutyrate 1.6 × 10-3M
Isoelectric point
pH 5.0±0.2
Unit Definition
One unit is defined as the amount of enzyme which converts 1 μ mole of 3?Hydroxybutylate to acetoacetate per minute at 37°C under the conditions specified in the assay procedure.
Optimum pH
8.5
Optimum temperature
45°C (Tris?HCl buffer)
Thermal stability
Stable at 37°C and below (pH 8.5, 10 mins)
Applications
Useful for enzymatic determination of ketone bodies
Synonyms
3-hydroxybutyrate dehydrogenase; 3-HBDH; NAD-β-hydroxybutyRate dehydrogenase; hydroxybutyRate oxidoreductase; β-hydroxybutyRate dehydrogenase; D-β-hydroxybutyRate dehydrogenase; D-3-hydroxybutyRate dehydrogenase; D-(–)-3-hydroxybutyRate dehydrogenase; β-hydroxybutyric acid dehydrogenase; 3-D-hydroxybutyRate dehydrogenase; β-hydroxybutyric dehydrogenase; EC 1.1.1.30; 9028-38-0
