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Cat No.
NATE-1138
Description
In enzymology, a 3alpha-hydroxysteroid dehydrogenase (B-specific) (EC 1.1.1.50) is an enzyme that catalyzes the chemical reaction: androsterone + NAD (P)+ ? 5alpha-androstane-3,17-dione + NAD (P)H + H+. The 3 substRates of this enzyme are androsterone, NAD+, and NADP+, whereas its 4 products are 5alpha-androstane-3,17-dione, NADH, NADPH, and H+. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor, more specifically it is part of the group of hydroxysteroid dehydrogenases.
Abbr
3α-HSD, Recombinant
Enzyme Commission Number
EC 1.1.1.50
Bio-activity
About 50U/mg powder
Appearance
White powder, lyophilized
Molecular Mass
About 28 kDa (SDS-PAGE detection)
Purification
90% (SDS-PAGE test)
Isoelectric point
4.8
Unit Definition
One unit will catalyze the oxidation of 1μmol of androsterone per min at pH8.9 at 25°C.
Optimum pH
7.0-9.0
Activators
EDTA
Inhibitors
Hg2+, Ag+
Applications
Bile acid is one of the substrates of 3α-hydroxy steroiddehydrogenase. 3α-hydroxy steroid dehydrogenase is used to catalyzethe dehydrogenation reaction of hydroxy steroid in clinic. So, HSD is used to detect the total bile acid clinically.
Synonyms
hydroxyprostaglandin dehydrogenase; 3α-hydroxysteroid oxidoreductase; sterognost 3α; 3α-hydroxysteroid dehydrogenase (B-specific); 3α-hydroxysteroid 3-dehydrogenase (B-specific); 3α-hydroxysteroid:NAD (P)+ 3-oxidoreductase (B-specific); EC 1.1.1.50
