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Cat No.
NATE-0012
Description
In enzymology, a L-ascorbate oxidase (EC 1.10.3.3) is an enzyme that catalyzes the chemical reaction:2 L-ascorbate + O2? 2 dehydroascorbate + 2 H2O. Thus, the two substRates of this enzyme are L-ascorbate and O2, whereas its two products are dehydroascorbate and H2O. This enzyme belongs to the family of oxidoreductases, specifically those acting on diphenols and related substances as donor with oxygen as acceptor. This enzyme participates in ascorbate metabolism. It employs one cofactor, copper.
Abbr
AAO, Native (Cucurbita sp.)
Alias
AAO; ascorbase
Species
Cucurbita sp.
Form
Lyophilized powder containing buffers and sucrose as stabilizer.
Enzyme Commission Number
EC 1.10.3.3
Bio-activity
1,000-3,000 units/mg protein
CAS No.
9029-44-1
Unit Definition
One unit will oxidize 1.0 μmole of L-ascorbate to dehydroascorbate per min at pH 5.6 at 25°C.
Applications
Ascorbate oxidase, from Cucurbita sp., may be used to study oxidative stress and heat stress response and tolerance. Ascorbate oxidase, from Creative Enzymes, has been used in ascorbic acid assays to study the heat stress response of Arabidopsis.
Synonyms
ascorbase; ascorbic acid oxidase; ascorbate oxidase; ascorbic oxidase; ascorbate dehydrogenase; L-ascorbic acid oxidase; AAO; L-ascorbate:O2 oxidoreductase; AA oxidase; EC 1.10.3.3; 9029-44-1; L-ascorbate oxidase
