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Cat No.
NATE-1104
Description
In enzymology, a D-lactate dehydrogenase is an enzyme that catalyzes the chemical reaction: (D)-lactate + 2 ferricytochrome c? pyruvate + 2 ferrocytochrome c. Thus, the two substrates of this enzyme are (D)-lactate and ferricytochrome c, whereas its two products are pyruvate and ferrocytochrome c. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with a cytochrome as acceptor. This enzyme participates in pyruvate metabolism. It employs one cofactor, FAD.
Abbr
D-LDH, Recombinant (Leuconostoc mesenteroides)
Alias
D-LDH
Species
Leuconostoc mesenteroides
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 1.1.1.28
Bio-activity
2085 U/mg protein at pH 7.0 and 25°C.
Molecular Mass
~ 36,455
Isoelectric point
~ 4.8
Unit Definition
One Unit of D-lactate dehydrogenase is defined as the amount of enzyme required to produce one μmole of NAD+ from NADH per minute.
Optimum pH
7
Thermal stability
up to 37°C
Stability
> 2 years at 4°C
Preparation Instructions
Swirl to mix the enzyme suspension immediately prior to use.
Applications
High purity recombinant D-Lactate dehydrogenase (L. mesenteroides) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Synonyms
EC 1.1.1.28; D-Lactic Dehydrogenase; 9028-36-8; (D)-lactate:ferricytochrome-c 2-oxidoreductase; lactic acid dehydrogenase; D-lactate (cytochrome) dehydrogenase; cytochrome-dependent D- (?)-lactate dehydrogenase; D-lactate-cytochrome c reductase; D- (?)-lactic cytochrome c reductase
