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Cat No.
NATE-0828
Description
DsbA appears to be necessary for correct formulation of disulfide bonds in exported proteins in vivo. DsbA is useful as a standard in immunoblotting. This protein catalyses the reduction and exchange of disulfide bonds and the oxidation of free sulfhydryl groups in vitro. It is the strongest oxidant of the thioredoxin superfamily. This thio/disulfide oxidoreductase is required for efficient disulfide bond formation in the periplasm of E. coli
Abbr
DsbA, Recombinant
Alias
DsbA
Source
E. coli
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Molecular Mass
23,149 Da
Purification
Greater than 95.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Stability
Lyophilized DsbA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution DsbA should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Applications
Western Blot
Synonyms
DsbA; Thiol:disulfide interchange protein dsbA; Disulfide Oxidoreductase
