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Cat No.
NATE-0208
Description
DT Diaphorase is a flavoenzyme that catalyzes the oxidation of reduced di-and triphosphopyridine nucleotides. It contains one mole of FAD per mole of enzyme. The enzyme found in rat liver catalyzes the oxidation of NADH and NADPH by various dyes and quinones. The molecular weight is found to be approximately 48 kDa Da. The pH optimum of the enzyme purified from rat liver is found to be 5.0. It is a cytosolic enzyme that catalyzes the two-electron reduction of various quinones. It catalyzes the conversion of vitamin K to vitamin K hydroquinone for utilization in the post-translational γ-glutamyl carboxylation reactions. These reactions are necessary for several proteins involved in blood coagulation.
Abbr
DTD, Recombinant (Human)
Alias
NQO1; QR1; DTD
Source
E. coli
Species
Human
Form
lyophilized powder
Enzyme Commission Number
EC 1.6.99.2
Molecular Mass
monomer mol wt 31 kDa
Unit Definition
One unit will reduce 1.0 μmole Cytochrome C per min/mg in the presence of menadione substrate at 37°C.
Applications
Human DT diaphorase has been used in a study to assess the development of novel quinone phosphorodiamidate prodrugs. Human DT diaphorase has also been used to investigate its crystal structure for the development of a model for its interaction with the cytotoxic prodrug 5-(aziridin-1-yl)-2,4-dinitrobenzamide (CB1954).
Gene Name
NQO1 NAD (P)H dehydrogenase, quinone 1 [Homo sapiens]
Synonyms
menadione reductase; phylloquinone reductase; quinone reductase; dehydrogenase, reduced nicotinamide adenine dinucleotide (phosphate, quinone); DT-diaphorase; flavoprotein NAD (P)H-quinone reductase; menadione oxidoreductase; NAD (P)H dehydrogenase; NAD (P)H menadione reductase; NAD (P)H-quinone dehydrogenase; NAD (P)H-quinone oxidoreductase; NAD (P)H: (quinone-acceptor)oxidoreductase; NAD (P)H:menadione oxidoreductase; NADH-menadione reductase; naphthoquinone reductase; p-benzoquinone reductase; reduced NAD (P)H dehydrogenase; viologen accepting pyridine nucleotide oxidoreductase; vitamin K reductase; diaphorase; reduced nicotinamide-adenine dinucleotide (phosphate) dehydrogenase; vitamin-K reductase; NAD (P)H2 dehydrogenase (quinone); NQO1; QR1; DTD; NAD (P)H: (quinone-acceptor) oxidoreductase; EC 1.6.99.2
GeneID
1728
mRNA Refseq
NM_000903
Protein Refseq
NP_000894
UniProt ID
P15559
Chromosome Location
16q12-q22
Pathway
Keap1-Nrf2 Pathway, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of amino acids and derivatives, organism-specific biosystem; Oxidative Stress, organism-specific biosystem; Regulation of ornithine decarboxylase (ODC), organism-specific biosystem
Function
NAD (P)H dehydrogenase (quinone) activity; coenzyme binding; cytochrome-b5 reductase activity; electron carrier activity; oxidoreductase activity; protein binding
