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Cat No.
NATE-0283
Description
Glycerol dehydrogenase is an enzyme in the oxidoreductase family that utilizes the NAD+ to catalyze the oxidation of glycerol to form glycerone (dihydroxyacetone).
Abbr
GDH, Native (Cellulomonas sp.)
Alias
GDH; GlDH; GlyDH
Species
Cellulomonas sp.
Form
Lyophilized powder containing bovine serum albumin
Enzyme Commission Number
EC 1.1.1.6
Bio-activity
50-125 units/mg protein
CAS No.
9028-14-2
Molecular Mass
mol wt ~390 kDa
pH Stability
pH 7.5 – 10.5 (25°C, 20hr)
Michaelis Constant
1.1 x 10ˉ2M (Glycerol), 8.9 x 10ˉ5M (NAD+)
Structure
10 subunits (42 kDa) per mol of enzyme
Isoelectric point
4.4 ± 0.1
Unit Definition
One unit will oxidize 1.0 μmole of glycerol to dihydroxyacetone per min at 25°C at pH 10.0.
Optimum pH
10.0 – 10.5
Optimum temperature
50°C
Thermal stability
below 55°C (pH 7.5, 15min)
Inhibitors
p-Chloromercuribenzoate, o-phenanthroline, monoiodoacetate, heavy metal ions (Co++, Ni++, Cu++, Zn++, Cd++)
Applications
This enzyme is useful for enzymatic determination of glycerol and of triglyceride when coupled with lipoprotein lipase in clinical analysis. Formation of NADH from the reaction of glycerol and NAD+ was catalyzed by the enzyme glycerol dehydrogenase.
Synonyms
EC 1.1.1.6; NAD+-linked glycerol dehydrogenase; glycerol:NAD+ 2-oxidoreductase; GDH; GlDH; GlyDH; 9028-14-2; glycerin dehydrogenase
