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Cat No.
NATE-0315
Description
In enzymology, a glycerol-3-phosphate oxidase (EC 1.1.3.21) is an enzyme that catalyzes the chemical reaction:sn-glycerol 3-phosphate + O2? glycerone phosphate + H2O2. Thus, the two substrates of this enzyme are sn-glycerol 3-phosphate and O2, whereas its two products are glycerone phosphate and H2O2. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. This enzyme participates in glycerophospholipid metabolism. It employs one cofactor, FAD.
Abbr
GPO, Native (Pediococcus sp.)
Alias
GPO
Species
Pediococcus sp.
Form
Lyophilized powder containing stabilizers
Enzyme Commission Number
EC 1.1.3.21
Bio-activity
40-80 units/mg solid
CAS No.
9046-28-0
Molecular Mass
mol wt ~76 kDa ((gel filtration))
pH Stability
pH 6.5-8.5 (25°C, 20hr) Thermal stability : below 40°C (pH 7.0, 15min)
Michaelis Constant
3.2x10-3M (L-α-Glycerophosphate), 6.8 x 10-3M (D, L-form)
Isoelectric point
4.1-/+0.1
Unit Definition
One unit will oxidize 1.0 μmole of L-glycerol 3-phosphate to dihydroxyacetone phosphate with the formation of H2O2 per min at 37°C, at the appropriate pH.
Optimum pH
Optimum temperature : 35-40°C
Inhibitors
Ionic detergents (SDS, LBS, etc.), Hg++, Ag+
Applications
This enzyme is useful for enzymatic determination of triglyceride when coupled with lipoprotein lipase (LPL-311, LPL-314) and glycerokinase (GYK-301, GYK-311) in clinical analysis.
Synonyms
EC 1.1.3.21; glycerol phosphate oxidase; glycerol-1-phosphate oxidase; glycerol phosphate oxidase; L-α-glycerophosphate oxidase; α-glycerophosphate oxidase; L-α-glycerol-3-phosphate oxidase; Glycerol 3-phosphate Oxidase; 9046-28-0; sn-Glycerol 3-phosphate:oxygen 2-oxidoreductase; L-Glycerol 3-phosphate Oxidase; GPO
