Cat No.
NATE-0994
Description
The Liberase T-Flex, Research Grade kit enables individual blends of collagenase and thermolysin in low level ratios. The enzymes are manufactured for applications in research where high purity, consistent quality and low levels of bacterial endotoxins are required for reproducible high cell yield and viability.
The Liberase T-Flex Research Grade kit contains highly purified collagenase class I and class II from Clostridium histolyticum. The two collagenase isoforms are blended in a precise ratio with each other. Highly purified thermolysin from Bacillus thermoproteolyticus is provided in a separate vial to allow individual adjustments of enzyme ratios for optimization of your tissue dissociation protocol.
Abbr
Liberase T-Flex
Bio-activity
Collagenase I/II: 2,895 U/bottle; Thermolysin: 180,000 U/bottle
Appearance
White lyophilized cake
Stability
At -15 to -25°C within specification range for 12 months.
Applications
Use Liberase T-Flex, Research Grade, for in vitro enzymatic dissociation of a broad range of tissues, enabling isolation of single cells. In particular intended for use in isolation procedures of pancreatic islets from several animal types.
Synonyms
Liberase; Liberase T-Flex