研究方向与目标
我组的研究兴趣主要围绕微生物生理和代谢方向。希望利用掌握的生理知识指导微生物的应用开发。我们一方面致力于新的的酶促反应和代谢途径的发现和功能分析，并在这个基础上对这些酶和代谢途径进行改进和优化。我们力图在整合的水平研究微生物功能，运用组学和分之间相互作用的数据来观察和理解微生物功能。
主要研究内容
1. 钙离子和相关调控蛋白对链霉菌发育的调控
2. 杰多霉素的合成机理、活性评价及应用开发
3. 青霉素扩环酶的改造和应用
4. 重要合成及调控复合物的功能分析
5. 梭菌的厌氧发酵产乙醇
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研究队伍
课题组长
杨克迁 博士 研究员
电话：010-64807459
电子邮件：yangkq@im.ac.cn
学历
1981-1885 青岛海洋大学海洋生物专业，学士
1985-1987 分别在四川外国语学院和北京外国语学院出国培训
1987-1993 加拿大达尔豪斯大学生物系，博士
研究工作经历
1993-1997加拿大达尔豪斯大学生物系Leo C. Vining实验室，博士后
1998-1999加拿大不列颠哥伦比亚大学Julian E. Davies实验室，博士后
1999-2001美国芝加哥FermaLogic Inc. 项目负责人，高级研究员
2001-2003 中国科学院微生物研究所，副研究员
2003-至今 研究员 博士生导师，微生物代谢工程中心副主任
研究领域
主要从事微生物生理代谢研究并运用相关知识改造微生物。
近年主持和参加的主要科研项目
院方向 棒酸的高通量筛选方法、蛋白质组和代谢分析 主持
基金 青霉素N扩环酶随机突变及底物专一性改造的研究 30170217 主持
基金 天蓝色链霉菌中钙调蛋白基因功能研究30400259 王胜兰 主持
基金 杰多霉素生物合成中氧化开环反应机理及新衍生物制备鉴30670017 范可强 主持
企业合作 棒酸产生菌的基因工程改造 主持
973 梭菌发酵过程中的蛋白质组和相互作用组分析 2007CB707801 参加
863 基于卤化酶基因筛选的海洋放线菌活性化合物的发现 2007AA09Z420 参加
863 未培养微生物基因资源的利用2001AA214141 参加
工作人员
王胜兰，博士，助理研究员
链霉菌生理代谢
wangsl@im.ac.cn
范可强，博士，助理研究员
链霉菌次生代谢合成途径
fankq@im.ac.cn
韩辉，实验师
微生物发酵
hanh@im.ac.cn
博士后
彭彦峰
pengyf@im.ac.cn
在读研究生
徐新平 2004级博士生
田秀云 2004级硕博生
王 娟 2005级硕博生
向四海 2006级博士生
于婷婷 2006级硕博生
殷 华 2007级博士生
潘国辉 2008级硕士生
客座研究生
李 佳 2006级硕士生
任晓慧 2006级硕士生
已毕业研究生
莫宏波 2000－2003，广东暨南大学
左 妍 2001－2004，香港科技大学
伍晓斌 2001－2005，美国林肯大学
陈义华 2001－2005，美国威斯康星大学
柳金满 2002－2005，北京生命科学研究所
石宣明 2002－2005，德国
吴 薇 2002－2006，美国
汪志军 2002－2006，上海交通大学
郑舰艇 2001－2007，美国马里兰大学
杨 旭 2004－2007，美国
蔺增曦 2005－2008，北京
已离开人员
贾翠娟 助理研究员，《微生物学通报》编辑部
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代表性论文
Linqi Wang#, Xiuyun Tian#, Juan Wang, Haihua Yang, Keqiang Fan, Gangming Xu, Keqian Yang*, and Huarong Tan*. (2009). Autoregulation of antibiotic biosynthesis: direct binding of jadomycin to an atypical response regulator. Proceedings of the National Academy of Sciences of the United States of America (#shared the first author) (accepted). (IF 9.598)
Xiaoyan Zhao, Shenglan Wang, Hai Pang, Keqian Yang, Bartlam Mark. Crystallization and preliminary X-ray diffraction studies of the calcium-binding protein CalD from Streptomyces coelicolor. Acta Crystallographica Section F: Structural Biology and Crystallization Communications. 2008, 64(9): 816-818. (IF 0.645)
Xinping Xu, Zhijun Wang, Keqiang Fan, Shenglan Wang, Cuijuan Jia, Hui Han, Eswar Ramalingam & Keqian Yang. 2008, Localization of the ActIII actinorhodin polyketide ketoreductase to the cellwall, FEMS Microbiology Letters. 287(1):15-21PDF (IF 2.274)
Fu DH, Jiang W, Zheng JT, Zhao GY, Li Y, Jiang JD, Yi H, Li ZR, Yang KQ, Wang YC, Si SY. Jadomycin B, an Aurora-B kinase inhibitor discovered through virtual screening. Molecular Cancer Therapeutics. 2008 7: 2386-2393. (IF 4.800)
Wang SL, Fan KQ, Yang X, Lin ZX, Xu XP, Yang KQ. CabC, an EF-hand calcium-binding protein, is involved in Ca2+ mediated regulation of spore germination and aerial hyphae formation in Streptomyces coelicolor. Journal of Bacteriology. 2008 190(11): 4061-4068. (IF 4.013)
杨旭，关东明，王胜兰，杨克迁 链霉菌真核型丝氨酸/苏氨酸蛋白激酶的研究进展 Wei Sheng Wu Xue Bao. 2008 48(1):126-131.
Yang KQ. TPR repeats and ELTR pattern: length variation as a function evolution mechanism. Wei Sheng Wu Xue Bao. 2007 47(6):956-962.
Zheng JT, Wang SL, Yang KQ. Engineering a regulatory region of jadomycin gene cluster to improve jadomycin B production in Streptomyces venezuelae. Applied Microbiology and Biotechnology. 2007 76(4):883-888
He YZ, Fan KQ, Jia CJ, Wang ZJ, Pan WB, Huang L, Yang KQ, Dong ZY. Characterization of a hyperthermostable Fe-superoxide dismutase from hot spring. Applied Microbiology and Biotechnology. 2007 75(2):367-376
Wang ZJ, Xu XP, Fan KQ, Jia CJ, Yang KQ. Sample preparation for two-dimensional blue native/SDS polyacrylamide gel electrophoresis in the identification of Streptomyces coelicolor cytoplasmic protein complexes. Journal of Biochemical and Biophysical Methods. 2007 70(4):565-572
Zhang L, Yan K, Zhang Y, Huang R, Bian J, Zheng C, Sun H, Chen Z, Sun N, An R, Min F, Zhao W, Zhuo Y, You J, Song Y, Yu Z, Liu Z, Yang KQ, Gao H, Dai H, Zhang X, Wang J, Fu C, Pei G, Liu J, Zhang S, Goodfellow M, Jiang Y, Kuai J, Zhou G, Chen X. High-throughput synergy screening identifies novel microbial metabolites as combination agent s for the treatment of fungal infections. Proceedings of the National Academy of Sciences of the United States of America. 2007 104(11):4606-4611
Cai Y, Chen XZ, Yang KQ, Huang L, Dong ZY. Construction and analysis of a metagenomic library from Tengchong hot spring soil in Yunnan Province. Wei Sheng Wu Xue Bao.2006 46(3):427-431.
Liu JM, Yang KQ. Functional analyses of TcrA-a TPR-containing regulatory protein in streptomyces coelicolor A3(2) Wei Sheng Wu Xue Bao.天蓝色链霉菌调控基因-tcrA功能的初步研究。微生物学报 2006 46(1):33-37.
Zheng JT, Rix U, Zhao L, Mattingly C, Adams V, Chen Q, Rohr J, Yang KQ. Cytotoxic activities of new jadomycin derivatives. The Journal of Antibiotics 2005 58(6):405-408
Shi XM, Luo YM, Zhang GF, Su ZG, Huang YB, Yang KQ. Analyses of Streptomyces coelicolor inner membrane proteome by multidimentional protein identification technology Chinese Journal of Biotechnology 2005 21(5):814-819.
Chen YH, Wang CC, Greenwell L, Rix U, Hoffmeister D, Vining LC, Rohr J, Yang KQ. Functional analyses of oxygenases in jadomycin biosynthesis and identification of JadH as a bifunctional oxygenase/dehydrase. Journal of Biological Chemistry. 2005 280(23): 22508-22514.
Wu XB, Fan KQ, Wang QH, Yang KQ. C-terminus mutations of Acremonium chrysogenum deacetoxy/deacetylcephalosporin C synthase with improved activity toward penicillin analogs. FEMS Microbiology Letters. 246(1):103-110.
Rix U, Wang C, Chen YH, Lipata FM, Remsing Rix LL, Greenwell LM, Vining LC, Yang KQ, Rohr J. The oxidative ring cleavage in jadomycin biosynthesis: a multistep oxygenation cascade in a biosynthetic black box. ChemBioChem. 2005 6(5):838-845*.
Zuo Y，Yang KQ. Construction and characterization of TetR and GFP fusion protein Chinese Journal of Biotechnology 2005 21(1):97-101
Mo HB, Bai LQ, Wang SL, Yang KQ. Construction of efficient conjugal plasmids between Escherichia coli and Streptomycetes. Chinese Journal of Biotechnology. 2004 20(5):662-666
Rix U, Zheng J, Remsing Rix LL, Greenwell L, Yang KQ, Rohr J. The dynamic structure of jadomycin B and the amino acid incorporation step of its biosynthesis. Journal of the American Chemical Society. 2004 126(14):4496-4497.
Yang KQ, Han L, He J, Wang L, Vining LC. A repressor-response regulator gene pair controlling jadomycin B production in Streptomyces venezuelae ISP5230. Gene. 2001 279(2):165-173.
Yang KQ. Prokaryotic calmodulins: new developments and evolutionary implications. Journal of Molelular Microbiology & Biotechnology. 2001 3(3):457-459.
Han L, Yang KQ, Kulowski K, Wendt-Pienkowski E, Hutchinson CR, Vining LC. A acyl-coenzyme A carboxylase encoding gene associated with jadomycin biosynthesis in Strepotmyces venezuelae ISP5230. Microbiology. 2000 146(Pt 4):903-910.
Iwagami SG, Yang KQ, Davies J. Purification of a protocatechuate 3,4-dioxygenase and subsequent characterization of a pca catabolic gene cluster from a Streptomyces sp. isolate 2065. Applied Environmental Microbiology. 2000 66(4):1499-1508.
Kulowski K, Wendt-Pienkowski E, Han L, Yang KQ, Vining LC, Hutchinson CR. Functional characterization of the jadI gene as a cyclase forming angucyclinones. Journal of the American Chemical Society. 1999 121(9):1786-1794.
Bateman KP, Thibault P, Yang KQ, White RL, Vining LC. Probing the substrate specificity of an enzyme catalyzing inactivation of Streptogramin B antibiotics using LC-MS and LC-MS/MS. Journal of Mass Spectrometry. 1997 32(10):1057-1063.
Bateman KP, Yang KQ, White RL, Thibault P, Vining LC. Inactivation of etamycin by a novel elimination mechanism in Streptomyces lividans. Journal of the American Chemical Society. 1996 118(23):5335-5338.
Facey SJ, Gross F, Vining LC, Yang KQ, van Pée KH. Cloning, sequencing and disruption of a bromoperoxidase-catalase gene in Streptomyces venezuelae, evidence that it is not required for chlorination in chloramphenicol biosynthesis. Microbiology. 1996 142(Pt 3): 657-665.
Yang K, Han L, Ayer SW, Vining LC. Accumulation of the angucycline antibiotic rabelomycin after disruption of an oxygenase gene in the jadomycin B biosynthetic gene cluster of Streptomyces venezuelae. Microbiology. 1996 142(Pt 1):123-132.
Mosher RH, Camp DJ, Yang KQ, Brown MP, Shaw WV, Vining LC. Inactivation of chloramphenicol by O-phosphorylation: a novel resistance mechanism in Streptomyces venezuelae ISP5230, a chloramphenicol producer. Journal of Biological Chemistry. 1995 270(45):27000-27006.
Yang KQ, Han L, Vining LC. Regulation of jadomycin B production in Streptomyces venezuelae ISP5230: involvement of a repressor gene jadR2. Journal of Bacteriology. 1995 177(21):6111-6117.
Han L, Yang KQ, Ramalingam E, Mosher RH, Vining LC. Cloning and characterization of polyketide synthase genes for jadomycin B biosynthesis in Streptomyces venezuelae ISP5230. Microbiology. 1994 140(Pt 12):3379-3389.
