Name: _____________________________________________________
To prepare an enzyme immobilisation and examine its application
Question 1
Explain what is meant by an immobilised enzyme:
_________________________________________________________________________________________________________________________________________________________________________________
Question 2
Give two benefits for using immobilised enzymes compared to free enzymes:
(i) ______________________________________________________________________________________________________________________
(ii)
______________________________________________________________________________________________________________________
Question 3
List two ways by which an enzyme may be immobilised:
(i) ________________________________________________________
(ii)________________________________________________________
Question 4
In preparing an enzyme immobilisation:
(i) Name the source of the enzyme that was immobilised:
___________________________________________________________
(ii) Name the enzyme.
___________________________________________________________
(iii) Why is sodium alginate used?
_________________________________________________________________________________________________________________________________________________________________________________
(iv) Why is calcium chloride used?
_________________________________________________________________________________________________________________________________________________________________________________
(v) What is the purpose of using a syringe?
_________________________________________________________________________________________________________________________________________________________________________________
(vi) Why are the beads left in the calcium chloride solution for a short period of time?
______________________________________________________________________________________________________________________
(vii) Why are the beads filtered and rinsed with water?
_________________________________________________________________________________________________________________________________________________________________________________
Application of the Immobilised Enzyme
Question 1
In examining the application of an immobilised enzyme:
(i) Name three pieces of equipment that were used:
______________________________________________________________________________________________________________________
(ii) Name the enzyme, substrate and product:
Enzyme: ___________________________________________________
Substrate: __________________________________________________
Product: ___________________________________________________
(iii) Why is warm water used in making the sucrose solution?
______________________________________________________________________________________________________________________
Glucose will collect in the beaker under the separating funnel.
(iv) How would you test for the presence of glucose?
_________________________________________________________________________________________________________________________________________________________________________________
(v) In which beaker will the glucose first appear?
______________________________________________________________________________________________________________________
(vi) State one advantage of using immobilised enzymes for this reaction:
_________________________________________________________________________________________________________________________________________________________________________________
Question 2
The separating funnels and their contents can be called bioreactors. What is a bioreactor?
_________________________________________________________________________________________________________________________________________________________________________________
